Doltchinkova Virjinia, R Angelova Plamena, Phytohemagglutinin and Light-Induced Charge Density Effects on Plasma Membrane of PlectonemaBoryanum, Journal of New Developments in Chemistry, Volume 1, Issue 1, 2014, Pages 11-19, ISSN 2377-2549, https://doi.org/10.14302/issn.2377-2549.jndc-13-262.
(https://oap-researcharticles.org/jndc/article/88)
Abstract: Effects of the plant lectin phytohemagglutinin M (PHA-M) and illumination on the electrophoretic mobility (EPM) and 90°-light scattering of suspension of spheroplasts obtained by lysis of the unicellular diazothrophic cyanobacteriumPlectonemaboryanumwere studied. Cells cultures were grown on media with different iron content: an iron-deficient (denominated as “Fe-starved” culture), an iron-sufficient (“Fe-sufficient”), and a twentyfold iron excess medium (“Fe-excess”). Lectin addition led to a decrease of the membrane charge density and aggregation of the vesicles. Our results indicate significant influence of the Fe2+ and illumination on the lectin-induced decreasing of EPM and light scattering intensity.
Keywords: Surface charge density; Plectonemaboryanum; Electrophoretic mobility; Light scattering; Spheroplasts; Phytohemagglutinin