Shahi Farshad, Rahimi Shabnam, Moradians Vahan, Jonaidi Jafari Nematollah, Izadi Morteza, Ozone/Oxygen Molecules Exert Mild Oxidative Stress on Testis Mitochondria Isolated from the Rat Testicular Ischemia/Reperfusion Injury, Journal of Antioxidant Activity, Volume 2, Issue 3, 2022, Pages 41-54, ISSN 2471-2140, https://doi.org/10.14302/issn.2471-2140.jaa-21-3911.
(https://oap-researcharticles.org/jaa/article/1837)
Abstract: Testicular ischemia-reperfusion injury is an urgent situation which needs a timely and precise diagnosis for prevention of testis damages. Here in we investigated ozone/oxygen therapy in Testicular Ischemia/Reperfusion Injury. For this purpose, animals (rats) were divided to four groups; control, torsion/detortion, torsion/detortion + ozone/oxygen (30 µg/ml) and only ozone/oxygen. Four hours after detorsion, in all groups orchiectomy was done -- for measuring the oxidative stress and mitochondrial toxicity parameters. Also, we preformed analysis of testicular spermatogenesis after 90 days. Our data showed that testicular torsion-detorsion induced significant increase in mitochondrial toxicity and decrease of spermatogenesis, malondialdehyde and GSSG levels were shown. Also, spermatogenesis, a remarkable decrease in malondialdehyde GSSG levels and mitochondrial toxicities were observed when compared with torsion-detorsion group. Obtained results for this research showed that ozone/oxygen therapy enhance antioxidant properties in the spermatogenic cells and protects testes from ischemia-reperfusion injury.
Keywords: Oxidative stress; Isolation of mitochondria; Testicular ischemia-reperfusion; cell death signaling.